Background:Inflammation of tonsils is a common disease of the throat that occurs predominantly in thechildren. Sothis prospective study was carried out to determine the pattern of bacterial strains and theirantibiotic sensitivity amongst children with tonsillitis admitted to the E.N.T Department, Faculty ofMedicine,Qena University Hospital for elective tonsillectomy.Patients and Method(s): Thirty-five children (2 to 17 years old) suffering from chronic tonsillitis weresampled. The bacterial isolates were identified phenotypically based on morphological, hemolysispattern and biochemical characters using different media or identified genotypically through 16S rRNAgene sequencing.Results: The bacterial strains were identified as Staphylococcus aureus (40%) followed byStreptococcus pyogenes (35%), Streptococcus australis (15%), and Streptococcus pneumoniae (10%).The susceptibility of the isolates was tested towards 8 different types of antibiotics (Bioanalyse®). Theresults showed that most bacterial isolates were multidrug-resistant bacteria that showed resistant tothree or more tested antibiotics.Conclusion: The present study revealed that the ß-haemolytic Streptococci (BHS) is a significant causalagent of tonsillitis in our environment which causes a potential danger of rheumatic fever andrheumatic heart disease.